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KMID : 0359319930330010131
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Korean Journal of Veterinary Research
1993 Volume.33 No. 1 p.131 ~ p.135
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Enzyme - linked immunosorbent assay for detection of bovine antibody to Brucella abortus
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Abstract
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Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis of Brucella abortus was developed and compared with plate agglutination test. Cell wall antigen was extracted from Brucella abontus 1119-3 by sonication and with a sodium deoxychlate solution. Optimum protein concentration of coating antigen were 0.4㎍/100μℓ protein on each microtiter plate well. Horse radish peroxidase (HRP) labeled protein-G was used as a tracer of reacted antibodies. ELISA confirmed the agreeable results of 40 cases out of 43 cases by plate aggulutination test. ELISA diagnosed positive cases 10 out of 12) and negative cases (1 out of 12) with dubious sera by plate agglutination test. From this results ELISA could be used for the early diagnostic tools of Brucellosis in cattle.
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